The Library of Alexandria as a research institution

According to the earliest source of information, the pseudepigraphic Letter of Aristeas, the library was initially organized by Demetrius of Phaleron, a student of Aristotle, under the reign of Ptolemy Soter.

Built in the Brucheion (Royal Quarter) in the style of Aristotle's Lyceum, adjacent to and in service of the Musaeum (a Greek Temple or "House of Muses", hence the term "museum"), the library comprised a Peripatos walk, gardens, a room for shared dining, a reading room, lecture halls and meeting rooms. However, the exact layout is not known. This model's influence may still be seen today in the layout of university campuses. The library itself is known to have had an acquisitions department (possibly built near the stacks, or for utility closer to the harbour), and a cataloguing department. The hall contained shelves for the collections of scrolls (as the books were at this time on papyrus scrolls), known as bibliothekai (βιβλιοθῆκαι). Carved into the wall above the shelves, a famous inscription read: The place of the cure of the soul.

The first known library of its kind to gather a serious collection of books from beyond its country's borders, the Library at Alexandria was charged with collecting all the world's knowledge. It did so through an aggressive and well-funded royal mandate involving trips to the book fairs of Rhodes and Athens and a (potentially apocryphal or exaggerated) policy of pulling the books off every ship that came into port. They kept the original texts and made copies to send back to their owners. This detail is informed by the fact that Alexandria, because of its man-made bidirectional port between the mainland and the Pharos island, welcomed trade from the East and West, and soon found itself the international hub for trade, as well as the leading producer of papyrus and, soon enough, books.

Other than collecting works from the past, the library was also home to a host of international scholars, well-patronized by the Ptolemaic dynasty with travel, lodging and stipends for their whole families. As a research institution, the library filled its stacks with new works in mathematics, astronomy, physics, natural sciences and other subjects. It was at the Library of Alexandria that the scientific method was first conceived and put into practice, and its empirical standards applied in one of the first and certainly strongest homes for serious textual criticism. As the same text often existed in several different versions, comparative textual criticism was crucial for ensuring their veracity. Once ascertained, canonical copies would then be made for scholars, royalty and wealthy bibliophiles the world over, this commerce bringing income to the library. The editors at the Library of Alexandria are especially well known for their work on Homeric texts. The more famous editors generally also held the title of head librarian. These included, among others,

Zenodotus (early third century BC)
Callimachus, (early third century BC), the first bibliographer and developer of the Pinakes - the first library catalog.
Apollonius of Rhodes (mid-third century BC)
Eratosthenes (late third century BC)
Aristophanes of Byzantium (early second century BC)
Aristarchus of Samothrace (late second century BC).